ABSTRACT
OBJECTIVE:To investigate clinical efficacy of intravenous drip of alprostadil combined with acupuncture in the treatment of diabetic foot ulcer and its effects on inflammatory factors levels and wound microvascular density. METHODS:A total of 72 patients with diabetic foot ulcer in our hospital during May 2014 to Sept. 2015 were divided into observation group and con-trol group according to random number table,with 36 cases in each group. Control group was given Alprostadil injection 10μg add-ed into 250 mL 0.9% Sodium chloride injection intravenously,qd,on the basis of routine treatment as insulin. Observation group was additionally given acupuncture therapy (Sanyinjiao,Zusanli,Fenglong,Yanglingquan,Yinlingquan and other acupuncture points),qd,on the basis of control group. Treatment courses of 2 groups lasted for 3 weeks. Clinical efficacy,wound healing time,fasting blood glucose,urine microalbumin,inflammatory factors,wound microvascular density as well as the occurrence of ADR were compared between 2 groups. RESULTS:The total response rate of observation group(91.67%)was significantly higher than that of control group(72.22%),and wound healing time was significantly shorter than control group,with statistical signifi-cance(P0.05). After treatment,above indexes of 2 groups were significantly lower than before,and urine microalbumin and inflammatory factors of the observation group was significantly lower than the control group,with statisti-cal significance (P<0.05). 14,21 d after treatment,wound microvessel density of observation group was significantly higher those of control group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Intrave-nous drip of Alprostadil combined with acupuncture can boost angiogenesis through mitigating inflammatory reactions,thus shorten the time of wound healing and enhance therapeutic efficacy.
ABSTRACT
Objective The paper described a convenient and sensitive radioirnmunoassav method which can be used to measure the amount of morphine in a single hair. Method Succinvl-eggalbumin-morphine was used as immunogen to immunize New Zealand rabbits for obtaining high quality antiserum. The morphine- labeled with Iodine-125 was purified by HPLC. The radioimmunoassay method was established and the amount of morphine in a single hair was detected. Results The affinity constant of antibody is 3.25 ? 10~11L/M. The radiochemical purity of I-mophine is 95% and the specific radioactivity is 112?Ci/?g. The detection limit for morphine (3SD from zero-dose binding) is 0.01ng/ml. Five normai person's single hair and five addict's were detected . The length of these specimens is 9-24cm and the weight of them 0.7- 2. 1mg. The amount of morphine in the hair of normai person is 1. 75 ? 0.37ng/mg (x ? 5) and that of the addictos'is 471 ?204ng/mg (x ? s). Conclusion This method can be employed for accurate mea-surement of the amount of morphine in a single hair and the result can be adopted to indicate the probability of illicit-drug use.